Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0001p246 | (1) | WCRB2014

Proteomic approach of cryo-damage in bovine spermatozoa

Yoon Sung-Jae , Kwon Woo-Sung , Rahman Saidur , Lee June-Sub , Park Yoo-Jin , You Young-Ah , Pang Myung-Geol

Cryopreservation is the most promising approach for storing spermatozoa. However, it could lead substantial damage to spermatozoa. We present here a couple of functional tests and 2DE PAGE proteome map of bovine spermatozoa in order to evaluate the effect of each cryopreservation procedure, e.g. fresh semen, after cooling, adding cryo-protectant (CP) and thawing on spermatozoa. Our result demonstrated that the cryopreservation procedure reduced the motility (%), viability (%) ...